Coding

Part:BBa_M11061

Designed by: Dan Nelson   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2009-04-13)


pfaD omega-3 polyunsaturated fatty acid synthase (mutated)

This is a single open reading frame which contains multiple genes that codes for necessary proteins in the sysnthesis of omega-3 polyunsaturated fatty acids. Ecosapentaenoic acid is the PUFA that is synthesized using this organism. The proteins coded within the gene are for dioxygenases related to 2-nitropropane dioxygenase activity. This gene is to be used in sequence with pfaA, pfaB, and pfaC. By cloning these genes into the same organism, it should be able to produce omega-3 fatty acids.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1024
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1396
    Illegal AgeI site found at 1614
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None